Abstract Objectives: Due to Pulmonary Artery Endothelial Cell (PAEC) dysfunction, Pulmonary Hypertension (PH) persists even after the Pulmonary Embolism (PE) has been relieved. However, the mechanism behind this remains unclear. Method: Here, the authors incubated Human PAECs (HPAECs) with thrombin to simulate the process of arterial thrombosis. Results: CCK8 results showed a decrease in the viability of HPAECs after thrombin incubation. In addition, the expression of Tissue Factor (TF), Monocyte Chemoattractant Protein 1 (MCP-1), VCAM-1, ICAM-1, cleaved cas-pase 3, cleaved caspase 9, and Bax protein were all increased after thrombin incubation, while Bcl-2 was decreased. The effects of 3-MA treatment further suggested that autophagy might mediate the partial protective effects of Resveratrol on HPAECs. To observe the effects of Resveratrol in vivo, the authors established a Chronic Thromboembolic Pulmonary Hypertension (CTEPH) model by repeatedly injecting autologous blood clots into a rat’s left jugular vein. The results exhibited that Mean Pulmonary Arterial Pressure (mPAP) and vessel Wall Area/ Total Area (WA/TA) ratio were both decreased after Resveratrol treatment. Moreover, Resveratrol could reduce the concentration and activity of TF, vWF, P-selectin, and promote these Superoxide Dismutase (SOD) in plasma. Western blot analysis of inflammation, platelet activation, autophagy, and apoptosis-associated proteins in pulmonary artery tissue validated the results in PHAECs. Conclusions: These findings suggested that reduced autophagy, increased oxidative stress, increased platelet activation, and increased inflammation were involved in CTEPH-induced HPAEC dysfunction and the development of PH, while Resveratrol could improve PAEC dysfunction and PH.

The microstructure and texture distribution of a Ti-1023 forged disk were investigated by scanning electron microscopy and synchrotron-based high-energy X-ray diffraction. The finite element method was used to simulate temperature and strain distribution in order to investigate the relationship of the α/β forging process with microstructure and texture distribution. A bimodal microstructure and rolling textures with large inhomogeneity were observed in the disk. A plate-like and a necklace-like morphology and volume fraction variations of the primary α phase were observed in different regions with different forging conditions, such as temperature, deformation, position, and high density of flow lines. Texture sharpness distribution of the β phase was in good agreement with the strain distribution, which suggests deformation may play the most important part in the texture inhomogeneity. A weak cube texture was obtained near the center of the disk, and fiber textures were found near the rim of the disk. The primary α phase also exhibited a transverse texture, which is favored by large deformation.